Thursday, December 1, 2022

Hepatitis B Surface Antibody Quantitative

Hepatitis B Blood Tests

Quantitative HBsAg and its Role in Chronic Hepatitis B Patient Management

The Hepatitis B Panel of Blood Tests

Only one sample of blood is needed for a hepatitis B blood test, but the Hepatitis B Panel includes three parts. All three test results are needed to fully understand whether a person is infected or not. Below is an explanation of the 3-part Hepatitis B Panel of blood test results.

  • HBsAg A positive or reactive HBsAg test result means that the person is infected with hepatitis B. This test can detect the actual presence of the hepatitis B virus in your blood. If a person tests positive, then further testing is needed to determine if this is a new acute infection or a chronic hepatitis B infection. A positive HBsAg test result means that you are infected and can spread the hepatitis B virus to others through your blood.
  • anti-HBs or HBsAb A positive or reactive anti-HBs test result indicates that a person is protected against the hepatitis B virus. This protection can be the result of receiving the hepatitis B vaccine or successfully recovering from a past hepatitis B infection. This test is not routinely included in blood bank screenings. A positive anti-HBs test result means you are immune and protected against the hepatitis B virus and cannot be infected. You are not infected and cannot spread hepatitis B to others.
  • Taking A Hepatitis B Test

    Testing for hepatitis B is performed on a sample of blood. A doctor, nurse, or other health care provider can obtain a blood sample using a small needle to draw blood from a vein.

    At-home hepatitis B testing requires that users carefully follow instructions provided in the test kit to collect a small sample of blood, package the sample, and mail it to a lab for testing.

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    Question 3 How Is The Quantitative Hepatitis B Surface Antibody Test Performed

    An immunometric technique is used. The anti-HBs binds to HBsAg ad and ay subtypes, which are coated on the test wells. Binding of a horseradish peroxidase-labeled HBsAg conjugate to the anti-HBs completes the sandwich formation. Unbound materials are then washed away. In the next step, the horseradish peroxidase catalyzes oxidation of a luminogenic substrate, producing light. Light signals are detected and quantified. Intensity of the light is proportional to the amount of anti-HBs present in the patient sample. The result is standardized to an international unit system and reported as milliinternational units per milliliter .

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    Understanding Of Lab Tests Results

    Please visit the site associated with The American Association for Clinical Chemistry for better understanding of tests. There you will find the most detailed and full information regarding lab tests. In common questions tab you will find answers on the most common questions.

    In addition, you can use a special form to ask the question. It is useful, if there is no answer on your question on the web site. A laboratory scientist will answer your question. It is a part of voluntary service provided by the American Society for Clinical Laboratory Science.

    Hepatitis B Surface Antibody

    What is the hepatitis B surface antibody?
    Hepatitis B Surface Antibody
    Description

    The Quantitative detection Hepatitis B virus Surface IgG antibody in human sera using the FDA approved Abbott ARCHITECT AUSAB-DIL test two-step chemiluminescent immunoassay.

    In the first step, sample, assay diluent, and recombinant Hepatitis B surface Antigen coated paramagnetic microparticles are combined. Anti-HBs present in the sample binds to the rHBsAg coated microparticles. In the second step, rHBsAg acridinium-labeled conjugate is added, which binds to IgG anti-HBs. Then pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units .

    A direct relationship exists between the amount of anti-HBs in the sample and the RLUs. The concentration of anti-HBs in the sample is determined using an active ARCHITECT AUSAB calibration curve. Results are reported as mIU/mL.

    For Batteries containing HBSAb see:

    Hepatitis B Antibodies , Quantitative detection of Hepatitis B virus Surface IgG antibody and Hepatitis B virus Core IgG and IgM antibodies

    Hepatitis B Battery , Quantitative detection of Hepatitis B virus Surface IgG antibody , Qualitative detection of Hepatitis B virus Surface Antigen and Qualitative detection of Hepatitis B virus Core IgG and IgM antibodies

    Hepatitis B Surface Antigen & Antibody , Quantitative detection of Hepatitis B virus Surface IgG antibody and Qualitative detection of Hepatitis B virus Surface Antigen

    Synonyms

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    Hepatitis B Surface Antibody Titer

    SKU:

    This antibody titer test checks for immunity to Hepatitis B.

    Hepatitis B Surface Antibody Titer This test measures your Hepatitis B Surface Antibody IgG titer level.

    Most people receive the Hepatitis B vaccine series when they are young and/or receive vaccine boosters as adults. As a consequence of either vaccination or prior exposure, people develop antibodies to Hepatitis B.

    The Hepatitis B Titer Test measures the Hepatitis B IgG antibody levels in your blood. Positive results mean that you are considered immune to Hepatitis B according to accepted international standards.

    Hepatitis B Titer Test results are reported as quantitative IgG titers.

    This information is for educational purposes only, and does not constitute medical advice, diagnosis or treatment in any way. This site does not replace the services of licensed health care professionals and all site users should consult with a physician regarding their health concerns.

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    Does Hepatitis B Show Up In Routine Blood Tests

    Routine blood tests do not detect hepatitis B virus infection. Hepatitis B tests are specifically done if blood tests show abnormal liver function results, or if a person experiences symptoms or falls into the high-risk category for HBV infection.

    A panel of HBV-specific blood tests are required to detect HBV infection.

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    Question 1 What Is The Clinical Indication For Hepatitis B Surface Antibody Quantitation

    Hepatitis B surface antibody quantitation is used to determine hepatitis B immune status, ie, to determine if the patient has developed immunity against the hepatitis B virus. Such immunity may develop following exposure to the hepatitis B virus or its vaccine.

    Patients at higher risk of exposure to the virus include:

    • Infants born to infected mothers
    • Sex partners of infected persons
    • People with more than 1 sex partner in the last 6 months
    • People with a history of sexually transmitted infection
    • Men who have sex with men
    • Injection drug users
    • Household contacts of an infected person
    • Healthcare and safety workers who have contact with blood and body fluids
    • People who have lived or traveled in an area in which hepatitis B is common
    • People who live or work in a prison

    Testing is not recommended routinely following vaccination. It is advised only for people whose subsequent clinical management depends on knowledge of their immune status. These people include:

    • Chronic hemodialysis patients
    • Immunocompromised people, including those with HIV infection, hematopoietic stem-cell transplant recipients, and people receiving chemotherapy
    • Infants born to women who test positive for the hepatitis B surface antigen
    • Sex partners of people who test positive for the hepatitis B surface antigen
    • Healthcare and public safety workers who have contact with blood or body fluids

    Negative But Other Hepatitis Tests Are Positive

    Hepatitis B Titer – Hep B Surface Antibody Test Results Overview

    Your HBsAb test may be negative even when other hepatitis B tests are positive, showing active or chronic infection. Further testing is necessary, especially for the hepatitis B surface antigen , which shows that the virus itself is circulating in your bloodstream and that you have an active or chronic infection.

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    What Do Hepatitis B Test Results Mean

    Hepatitis B test results help determine if HBV infection is negative or positive, and if positive, whether the infection is acute or chronic, or if recovery is complete. A combination of results are considered to identify and classify HBV infection status.

    The following are some interpretations of hepatitis B test results:

    Table: Hepatitis B test results and interpretations

    Test

    Can I Take The Test At Home

    Samples for hepatitis B testing can be collected at home. At-home hepatitis B testing requires a patient to collect a blood sample, typically from a fingerstick using a very small needle provided in the test kit. Once a blood sample is collected, it is prepared according to the instructions contained in the test kit and mailed to a laboratory for testing.

    Because there are numerous types of tests for HBV, it is important to look closely at the specific components of any at-home test kit. Many at-home test kits only look for hepatitis B surface antigen .

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    Hepatitis B Surface Antibody Qualitative

    Test Code: 499

    Methodology: Immunoassay

    Clinical Significance: The detection of anti-HBs is indicative of a prior immunologic exposure to the antigen or vaccine. To determine immune status as 10 mIU/mL as per CDC guidelines, please order Hepatitis B Surface Antibody, Quantitative.

    Alternative Name: Anti-HBS Anti-HBS Qual Anti-HBSAG Australian Antibody HB Surface Ab

    Supply: T01 – Red/Gray SST 8.5mL

    Preferred Specimen: Serum

    Transport Container: Serum Separator Tube

    Transport Temperature: Room Temperature

    Specimen Stability: Room Temperature: 5 days

    Rejection Criteria: Gross Hemolysis, Gross Lipemia

    For additional supply or collection device information, please contact DLO’s Customer Service at 891-2917, option 2.

    The information contained here on the Diagnostic Laboratory of Oklahoma website is not to be construed as medical recommendations or professional advice. Neither DLO nor its affiliates, agents or any other party involved in the preparation or publication of the works presented is responsible for any errors or omissions in information from the use of such information. Readers are encouraged to confirm the information contained herein with other reliable sources and to direct any questions concerning personal health care to licensed physicians or other appropriate health care professionals.

    Question 5 What Is The Natural History Of Hepatitis B Surface Antibody During Acute Hepatitis B Infection And Convalescence

    Persistence of hepatitis B surface antibody and immune ...

    HBsAg can be detected in the blood 4 to 10 weeks after exposure. This corresponds to onset of symptoms and viremia detectable by nucleic acid amplification methods. Most hepatitis B infections are self-limited and are associated with disappearance of HBsAg within 4 weeks of onset of symptoms. The anti-HBs then appears and increases to a plateau level that persists indefinitely.2

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    How To Get Tested

    Hepatitis B testing is typically prescribed by a doctor and performed in a hospital, lab, or other medical setting. Taking a hepatitis B test requires a blood sample, which can be collected by a health care professional.

    For laboratory-based testing, blood is drawn from a patients vein. After blood is collected, the sample is sent to a laboratory for analysis.

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    Discusses Physiology Pathophysiology And General Clinical Aspects As They Relate To A Laboratory Test

    Hepatitis B virus infection, also known as serum hepatitis, is endemic throughout the world. The infection is spread primarily through blood transfusion or percutaneous contact with infected blood products, such as sharing of needles among injection drug users. The virus is also found in virtually every type of human body fluid and has been known to be spread through oral and genital contact. HBV can be transmitted from mother to child during delivery through contact with blood and vaginal secretions, but it is not commonly transmitted via the transplacental route.

    The incubation period for HBV infection averages 60 to 90 days . Common symptoms include malaise, fever, gastroenteritis, and jaundice . After acute infection, HBV infection becomes chronic in 30% to 90% of infected children younger than 5 years of age and in 5% to 10% of infected individuals age 5 or older. Some of these chronic carriers are asymptomatic, while others progress to chronic liver disease, including cirrhosis and hepatocellular carcinoma.

    Hepatitis B surface antigen is the first serologic marker, appearing in the serum 6 to 16 weeks following HBV infection. In acute cases, HBsAg usually disappears 1 to 2 months after the onset of symptoms with the appearance of hepatitis B surface antibody . Anti-HBs also appears as the immune response following hepatitis B vaccination.

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    What Is A Hepatitis B Surface Antibody Test

    Hepatitis B surface antibody test is part of a panel of blood tests to diagnose HBV infection. Hepatitis B surface antibody test determines the presence and quantity of anti-HBs in the blood serum, which can indicate protection from HBV infection.

    Hepatitis B disease affects the liver and commonly spreads through body fluids such as blood, semen, and vaginal secretions.

    Question 7 Is Hepatitis B Surface Antibody Antibody Always Acquired After A Completed Vaccination Protocol

    Quantitative HBsAg and its Role in Chronic Hepatitis B Patient Management

    No. After 3 intramuscular doses of vaccine, > 90% of healthy adults and > 95% of those < 19 years of age develop immunity .1 However, there is an age-specific decline in development of immunity. After age 40 years, about 90% of people become immune, but by age 60 years, only 75% of people become immune.1 Larger vaccine doses or an increased number of doses are required to induce immunity in many hemodialysis patients and in other immunocompromised people.1

    References

  • Centers for Disease Control and Prevention. Hepatitis B. . Accessed May 30, 2013.
  • Ganem D, Prince AM. Hepatitis B virus infectionnatural history and clinical consequences. N Engl J Med. 2004 350:1118-1129.
  • This FAQ is provided for informational purposes only and is not intended as medical advice. A clinicians test selection and interpretation, diagnosis, and patient management decisions should be based on his/her education, clinical expertise, and assessment of the patient.Document FAQS.105 Revision: 0

    Recommended Reading: Hepatitis B Is More Infectious Than Hiv

    What Is The Purpose Of A Hepatitis B Test

    Hepatitis B test is performed to detect, classify, and treat hepatitis B virus infection.

    Hepatitis B blood tests involve the measurement of several HBV-specific antigens and antibodies. In addition, HBV blood tests also include liver enzymes and liver function tests to assess and monitor the condition of the liver and provide appropriate treatment.

    The HBV specific tests include the following:

    • HBsAg: HBsAg is an antigen found on the surface of hepatitis B virus. HBsAg may be detected in the blood any time after 1 week post-exposure to HB virus, but usually appears after 4 weeks.
    • Anti-HBs: Anti-HBs are antibodies produced by the bodys immune system to fight HBsAg. Anti-HBs from a prior infection or vaccination provides immunity against further infection.
    • Hepatitis B core antigen : HBcAg is an antigen found in the core layer which covers the hepatitis B viral DNA.
    • Hepatitis B core antibody : Anti-HBc is the antibody that fights HBcAg. Anti-HBc is the first detectable antibody after HBV infection. There are two kinds of Anti-HBc:
    • Immunoglobulin M hepatitis B core antibody : IgM anti-HBc indicates acute or reactivated recent infection within the previous 6 months.
    • Immunoglobulin G hepatitis B core antibody : IgG anti-HBc may indicate previous or chronic infection. Once present, IgG anti-HBc persists for a lifetime.
  • HBV DNA: HBV DNA is the genetic material of the HB virus and this test is a measure of the actual viral load and replication.
  • What Is The Normal Range For Hepatitis B Surface Antibody

  • What Is the Normal Range for Hepatitis B Surface Antibody? Center
  • Hepatitis B surface antibodies are measured in blood samples in milli-International Units/milliliter mIU/mL). The ranges for hepatitis B surface antibodies are:

    • Anti-HBs greater than 10-12 mIU/mL: Protected against hepatitis B virus infection, either from vaccination or successful recovery from a previous HBV infection.
    • Anti-HBs less than 5 mIU/mL: Negative for HBV infection, but susceptible and hence requires vaccination.
    • Anti-HBs from 5-12 mIU/mL: Inconclusive results and the test should be repeated.

    However, there is no standardization of these values so it is advisable to check the manufacturers values it is the reason values are mainly reported as positive or negative.

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    Hep B Titer Test Required By Most Schools And Employers

    This assay is used to determine immune status for Hepatitis B.

    Hepatitis B Surface Antibody : The surface antibody is formed in response to the hepatitis B virus. Your body can make this antibody if you have been vaccinated, or if you have recovered from a hepatitis B infection. If this test is positive, then your immune system has successfully developed a protective antibody against the hepatitis B virus. This will provide long-term protection against future hepatitis B infection. Someone who is surface antibody positive is not infected, and cannot pass the virus on to others.

    This is a Quantitative test required by many schools and medical programs. Levels of anti-HBs will be provided.

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